You may think that you should list your items on Ebay on Saturday mornings because that is when you have the most free time or on Monday nights after 10:00 because the family is in bed and you finally have total concentration. You need to ask yourself just how many possible buyers will be at their computers at closing time. Especially when 10:00 p.m. on the West Coast is 1:00 a.m. on the East Coast. There are lots of possible buyers shopping at lunchtime during the week. But, because of the enormous amount of traffic at that time, you really cut down on the possibility of last-minute bidding if the bids are all in an internet traffic jam and can't get through at all.
The amount of bidders available at closing time is of utmost importance to your auction. Most of the bidding on any auction will occur in the first 24 hours (when it is listed as new) and on the last day (when is listed as ending today), with most people only looking at one of these two times. And, the bulk of the serious bidding will be in the last 5 minutes! An auction is truly at its best when two or more people fight over your item, and they may not be awake to do so at 1:00 in the morning.
When to list your items is best decided on actual sales histories of your particular items. Hardware and tools may get more male bidders who are looking on Saturday afternoon, and pokemon cards may get more attention from kids right after school. Etc. So, check the sales of completed items. You should be able to see a definite pattern for each type of item you plan to list. This is the first step.
If your item sells best on a weekday evening, now you need to see if there is also a second pattern. It sold best on Tuesday one week and Thursday the next week. Why? One big factor in evening sales now is "Who Wants to be a Millionaire?" There are almost no bidders at their computers when this show is on television. This show happens to be moved around from week to week. On top of that, if it is on at 8:00 p.m., that means 8:00 p.m. on both coasts so West Coast seller has to work around 5:00 p.m. through 8:00 p.m., and the East Coast seller has to work around 8:00 p.m. through 11:00 p.m.
There are many more factors that come into play. Watch the weather forecasts for all parts of the country. If the East Coast is due for heavy rains, more possible bidders will be available at their computers instead of outside enjoying the spring or summer weather - and vice versa. If an area is in the middle of a terrible heat wave, they may be hiding inside at their computer instead of doing anything outside. Great weather is bad for sales. You may not want to have items close on Thanksgiving afternoon when most of the country is sitting down to a great meal - or in the evening on the day of a Presidential election. Contrary to this logic, Christmas morning may be a good time to have items close because many people don't have family with whom to share their day or they are not of a Christian faith. Those people would see this as a great time to sneak in a few bids. Other people may go shopping on Christmas afternoon to try to buy what they didn't receive for a gift!
Selling at the busiest time of the day will make your photos load slower. At any time, using a dial-up connection puts your auctions at risk since they will take longer to load. And, since most dial-up connections get lost often, there is always the chance that your photos will not load at all. When either happens, you will lose buyers. Some could bookmark your auction to try again later, but last-minute shoppers don't have time to come back. Cable modem or DSL will make everything you do on the internet at least 10 times faster - without disconnects. You only need to be have your photos not load during the last half hour of an auction once to know how important your internet connection is.
The real answer to when to list your items is it is totally relative….relative to as many factors as you can think of. You will need to do research on the past and try to foresee the future. Good luck on the latter.